MOSA - Medical Oxygen Society of the Americas

The progress of today medical science is oriented to development of new technologies allowing not only highly effective treating diseases, but also preventing them, increasing the vital potential of the organism. One of such progressive approaches is ozone therapy. It has a wide range of action in surgery, dermatology, urology, internal medicine.

It would be strange if ozone would not be put into use in cosmetology - one of the most progressive fields of its application. In methods of treatment with ozone a particular attention is paid to improvement of microcirculation, oxygenation, energy supply of cells, proper trophism and protection of skin.

Many cosmetic problems - acne, teleangiectatic rosacea, outflowing of the hair, wrinkles, allergy, cellulites, fatness - are reflecting the internal state of the organism, therefore they should be treated not only from the outside, but also inside. And this task can be successfully done by ozone producing a detoxication, antimicrobial, antiviral effect and increasing the organism’s immunity.

Ozone is a unique agent preserving and restoring the natural beauty and health of skin. Ozone therapy does not mask defects of skin, but normalizes its natural functions and stimulates its own work.

The mechanism of action of ozone can be demonstrated on the example of its use within a complex program for skin rejuvenescence.

Aging at the level of epidermis is induced by inhibition of proliferous cell division, exhaustion of epidermis, increase in keratin layer, decrease in elasticity and increase in residual epidermis deformation (”crow’s-foot”), formation of deeper wrinkles.

Skin functions properly and looks young only in case of proper trophism and division of epidermis cells. The natural mechanism of cell trophism is realized by means of tissue liquid, lymph and blood plasma.

The functional efficiency of this network and quality of epidermis trophism are considerably decreased under the influence of negative factors of environment. Aging at the level of dermis is induced by changes in the structure of collagenous and elastic fibers. On exposure of collagen to free radicals it comes to “together-sewing” of collagen, therefore collagenase enzyme responsible for collagen degradation in normal conditions is not able to realize its function.

It comes to accumulation of pathologically modified collagenous fibers, a decrease in their water-retaining ability and in the total level of water in dermis. It comes to a functional disturbance of hyaluronidase, the specific enzyme producing an effect on hyaluronic acid and defining skin penetrance.

Owing to aging it comes to a decrease in the activity of some key enzymes of respiratory chain and intensity of cytochromoxidase synthesis that leads to energy deficiency in cells. It comes to inhibition of epidermis cell regeneration, exhaustion and fragmentation of elastin fibers, and a result of all these pathological processes is formation of wrinkles.

Skin aging can be inhibited, and skin structure can be restored through neutralization of the above-mentioned pathological processes thereby eliminating the reason, but not only the consequence - formation of wrinkles. Exactly this is the target of ozone therapy.

Ozone is a substance created by the nature, produces a complex, integral effect on the human body. For treatment of “problem” skin and wrinkles it is recommended to use the methods of local and systemic ozone therapy: by local route - subcutaneous ozone injections and ozonized olive oil in the form of face packs and for massage; by systemic route - intravenous drop-by-drop infusions (drips) of ozonated solutions, major and minor autohaemotherapy with ozone, rectal ozone insufflations. The approach to treatment and choice of methods are strictly individual for each patient.

Ozone introduced subcutaneously activates metabolic processes in macroergic cells, normalizes active membrane transport (K-Na pump), penetrance, deformability, viscosity and electric properties of membranes. At the same time, it comes to an increase in the intensity of energy processes: on the one hand, through the increased utilization of oxygen by cells owing to activation of aerobic glycolysis, Krebs’ cycle, b-oxidation of fatty acids, and on the other hand - through optimization of the oxygen-transport function of blood:

- At presence of ozone the erythrocytes are able to bond and transfer 10 times more oxygen and more easier release it to tissues.

- Besides, it comes to stopping of “oxidative stress”, activation of the antioxidant defense system and neutralization of the destroying effect of free radicals.

- it comes to stimulation of protein synthesis including its own collagen and elastin, an increase in the regenerative potential of proliferative skin layer.

- It comes to an increase in tissue liquid volume in deep layers and restoration of the natural skin ability to retain water, and as a result.

- It comes to ironing and disappearance of “crow’s-foot”, deeper wrinkles, and the rejuvenescence effect is well manifested.

- The essential difference of ozone therapy from other cosmetic remedies and procedures is its dual action from the outside and inside of the organism that leads to normalization of trophism, oxygenation, moistening, protection and exchange restoration of skin.

- It helps to achieve general cleaning, sanation and tonization, but not only a temporary cosmetic effect.

- Thus, ozone therapy is a revolutionary remedy for rejuvenescence allowing to restore the barrier, immune, water-retaining, reparative and separative functions of skin as well as to improve its structure.

Especially noticeable and fast results through ozone therapy are observed in patients with diagnosis “skin stress”.

The most frequent method of administration of ozone for rejuvenescence is by intravenous drop-by-drop route and in the form of ozone bath “torso”. This is convenient as during the ozonization the cosmetologist can perform other procedures on face, neck, decollete that saves the patient’s time.

For the organism’s cleaning, elimination of roughages and toxins including stress toxins producing a negative effect on skin the complex treatment of skin fading contains global intestinal cleaning. On completing this procedure it is recommended to perform rectal ozone insufflation thereby creating beneficial conditions for regeneration of “healthy” intestinal flora and producing a beneficial effect on the whole organism.

For correction of available signs of skin fading after 35 years of great benefit can be the rejuvenescence courses of ozone therapy in the form of subcutaneous injections at the places of wrinkle localization and other places with manifested skin fading (chin, cheeks, neck).

After the procedure it is recommended to perform manual massage - plastithermic or cosmetic for equal distribution of ozone-oxygen gas mixture.

The well-manifested effect is observed already after 3-4 procedures: ironing of fine wrinkles, an increase in skin turgor, a decrease in face pastosity, improvement of face color, appearance of glow.

The patients report about a decrease in skin dryness, disappearance of skin constriction sensation even after washing. Both the patients and doctors notice a lifting effect in submaxillary and cheen-chin areas. And what is very important is improvement of general feeling.

Ozone therapy is used in the form of local ozone injections onto inflammatory areas (infiltrates, pustular elements). In some cases it is recommended to perform primary focal sanation by method of electrocoagulation, opening and elimination of purulent content, and then ozone therapy.

The depth and number of injections under one focus depends on the size of inflammatory element. Up to 5 ml of ozone-oxygen gas mixture is injected into each point.On average one treatment course consists of 5-6 procedures at an interval of 5 days.

All patients show clinical improvement already after one procedure. This manifests in infiltrate softening, a decrease in swelling, exudation, hyperemia, painfulness. Treatment time is reduced by 2-3 times as compared with traditional methods.

Any complications and side-effects are not observed. In case of torpid course of acne disease, diffused process the most effective method is autohaemotherapy with ozone considerably increasing the immunity and producing an antibacterial effect.

O3 Ozone is also an integral part of postoperative rehabilitation for prevention of complications. Ozone helps to intensify lymphodrainage, restore microcirculation, improve tissue trophism, stimulate metabolic processes. The effect is noticeable already after one-two procedures: a decrease in edema, tissue thickening and painfulness.

After 4-5 procedures it comes to restoration of skin sensitivity, a decrease in hyperemia and disappearance of constriction sensation in the area of postoperative sutures. More comfortable sensations of the patient have a positive influence on his evaluation of results of the performed operation.

After the wide practical introduction of such methods as middle and deep chemical peelings, laser and mechanical skin grinding, injection contour plasty we are facing a serious problem - hypertrophic scars.

Ozone gas injections around the scar induce disappearance of skin constriction sensation, paling and softening of scars and their further resorption. As a result, it comes to significant ironing of scar surface, restoration of tissue elasticity.

Obviously, ozone therapy in the form of injections allows solving many serious problems arising in the practice of dermatocosmetologist. Of great importance is the fact that the result of similar procedures is not only solution of esthetic problems being a reason for a visit to doctor, but also improvement in general feeling of patients through the many-sided non-specific effect of ozone on tissues and the organism as a whole.

Edematous-fibrosclerotic panniculopathy or cellulites is an exclusively female problem and represents a pathological state of fat cells (adipocytes). Among the reasons and risk factors inducing a change of fatty tissue are numbered genetic, family, national features, pubescence period, pregnancy and delivery, hormonal disturbances and intake of contraceptive preparations, age and life way.

According to various sources, 80% to 95% of women are suffering from cellulites. The affected areas are thighs and buttocks, more rarely - back and arms.

The initial stage of cellulites is characterized by microcirculatory disorder, swelling, stagnation of liquid in subcutaneous tissue. So, it comes to edematous form of cellulites that in case of further progression is transformed to fibrosclerotic form.

Fatty tissue fulfills a function of energy depot in the organism. Each fat cell is closely surrounded by capillary network, therefore it is in continuous and close contact with blood.

In healthy fatty tissues the adipocytes receive a sufficient quantity of oxygen and nutritious substances, and products of cell activity are removed via cell membrane and taken away by blood flow.

A disturbance of this mechanism leads to development of local hypoxia, toxicosis and acidosis and in further to cellulites.

In conditions of oxygen deficit in acidic environment it comes to active growth of connective tissue that surrounds fat cells by membrane and forms cellulites small knots (micronodules). Fat cells degenerate, make groups and form in connective tissue thick conglomerates (macronodules) that block circulation and lymphatic flow, it comes to calcination of fat cells.

Edematous-fibrosclerotic panniculopathy is characterized by development of microangiopathies and disturbances of lymphatic structure manifested as lymphangiectasia and lymph edema. On the initial stage of cellulites a cosmetic effect in skin is manifested in the form of insignificant hilliness, the so-called “orange peel” that in case of further progression is transformed to “grape bunches”.

Thus, the main mechanism of cellulites development is microcirculatory disorder that leads to edema, which in turn aggravates hemodynamics closing circulus vitiosus. Cellulites treatment should be focused:

? � Secondly, on correction of pathogenetic mechanisms of disease development; ?�

It is mostly rational to start cellulites treatment on early stages when it only represents an esthetic problem. In this case ozone can be used as a monotherapy, and it takes a few procedures in the form of subcutaneous ozone injections to achieve a good cosmetic effect.

The program of cellulites therapy combines local ozone therapy (subcutaneous ozone injections, massage and wrapping on ozonized olive oil) and systemic ozone therapy (intravenous infusion of ozonated solutions, patent ’s autoblood) that provides a more noticeable and stable result.

The parenteral methods restore hormonal background, protein, fat and carbohydrate metabolism, quickly remove consequences of stress and chronic tiredness, balance the processes of excitation and inhibition of nervous system, increase oxygen supply of the whole organism.

Treatment variants should be determined individually depending on the stage and form of cellulites, volume of affected surface, age, associated pathology, individual features of the organism (pain sensitivity etc.). The usual course of therapy includes a certain number of sessions (from 10 to 20) with frequency 1-3 times a week. Gas is introduced subcutaneously by means of special microneedles with length of 4 or 13 mm.

Positive results of treatment are observed already after 3-5 sessions and well manifested, the patients start to notice lightness in the lower limbs and a decrease in pastosity and edema. On the next stage it comes to reduction or complete disappearance of “orange peel”, a decrease in volumes of subcutaneous tissue.

Through ozone therapy it comes to a fast-progressive decrease in fat layers, fatty tissue becomes more thick and compact. Good results can be also achieved in the formation of body contours (nonsurgical modeling of figure - nonsurgical liposuction): correction of double chin, face oval, forms of breast, thighs and buttocks.

Ozone facilitates fast elimination of fat from stomach, particularly from its lower part that undergoes a little change through traditional methods of correction and practically no change through diet therapy and general slimming.

The essential difference of ozone therapy from other forms of lipolysis (mechanical, electric, ultrasound, chemical) is that ozonolipolysis or ozonolysis (destruction of fatty tissue by ozone) combines both a local and systemic detoxication effect, intensifies fat splitting and utilization.

- It comes to stimulation of cellular processes, an increase in the oxygen-transport function of blood and accordingly the oxidoreduction potential of the organism.

- Ozone introduced by parenteral route does not only activate lipid exchange, but also initiates the mechanism of natural processing of the organism’s own energy resources i.e. fat.

- Ozone stimulates b-oxidation of fatty acids, energy production and activity of the hepatocytes focused on processing of lipid fractions; on this way liver is not affected.

- Under the action of ozone in the hepatocytes it comes to activation of structural-functional mechanisms of transformation of fat energy substrates to carbohydrate ones.

- Ozonization induces production of catalase and peroxides involved in the catabolism of fatty acids and synthesis of glycogen and glucose from products of this catabolism i.e. ozone performs the antioxidant and detoxication functions.

- The anticellulites effect and slimming are realized on a background of tonus restoration, strengthening and tonization of all tissues exposed to ozone therapy.

- Skin is not hanging like after usual slimming and other methods of cellulites treatment, on the contrary - it becomes more elastic, its appearance improves.

- It comes to normalization of connective tissue structure as ozone has a fibrinolytic effect, a decrease in the level of hypertrophic adipocytes and metabolism restoration in subcutaneous tissue.

- As a rule, cellulites is not an independent pathology, but manifests on a background of the main disease owing to the organism’s intoxication. Therefore, without removing a reason it is impossible to cure a consequence.

- Ozone producing a complex effect on the organism acts on the molecular, cellular, systemic levels and treats both actual diseases and their consequence i.e. cellulites.

- Ozone therapy provides remission for half to one year, but not only 1-2 months like traditional treatment.

- So, ozone is used to treat cellulites, but not only to achieve a temporary cosmetic effect.

- On the results of numerous clinical investigations it can be claimed that for today ozone therapy is one of the most effective methods for prevention and treatment of cellulites.

- The gathered experience allows making a conclusion that ozone therapy is characterized by simplicity of application, good tolerance by patients, practically complete absence of side-effects, high efficiency and reasonable prices for appropriate equipment.

Celulitis is a disease due to alterations on the connective tissue. The adipocytes degenerate, growing hard, causing fiber-tissue to appear. "Orange-skin" is a celulitis characteristic, vascular weakness is another because this problem appears, in addition to other causes, due to a bad vein or limphatic flow.

Giving a mixture of oxygen/ozone in the celulitis zone causes a very important improvement in the peripherical circulation. As well, ozone is able to destroy the degenerated fat tissue (very important to fight against this problem).

HBOT / O2 Oxygen Provides Dramatic Improvements for Cosmetic Surgeons

Cosmetic Surgeons and Oxygen Therapy Hyperbaric (HBOT).

Hyperbaric oxygen can: Help resolve unexpected complications post-surgery. Dramatically reduce post-surgical bruising, swelling, and inflammation. Speed up healing and recovery time from elective surgery. Important effects of post-surgical hyperbaric oxygen for your patients would be the stimulation of leukocyte microbial killing, the enhancement of fibroblast replication, and increased collagen formation and neovascularization of ischemic tissue.

Hyperbaric oxygen is a safe way to alter the inflammatory process to help wound healing. It cuts down pain and swelling, inflammation in tissues, and it has an anti-bacterial effect. Patients who had this therapy in conjunction with their cosmetic surgery seem to have a shorter recuperation period. And scars have been shown to heal better with hyperbaric oxygen therapy.

Cosmetic surgeons are recommending hyperbaric oxygen particularly for patients who have problems with healing. They may have been smokers, overweight, they may be immunity suppressed, or their incisions may have become infected, etc. The bruising goes away faster, swelling decreases faster, and the patients actually feel better overall.

HBOT is known to be exceedingly effective for laser peels. After a laser peel, lingering redness is a problem for many patients. The chamber gets rid of the red more quickly. For liposuction, the chamber helps the swelling decrease much faster, which results in less post-operative discomfort. Normally, only red blood cells are fully saturated with oxygen molecules and the body consumes energy sending it to demanding cells.

However, inside the hyperbaric oxygen chamber, oxygen obeys Henry’s Universal Gas Law and so the oxygen becomes soluble using passive diffusion to saturate the plasma and cerebrospinal fluid. Depending on the treatment depth, the patient will leave the chamber saturated with up to twenty times normal oxygen levels.

HBOT triggers many positive physiological events:

* New tissue striving to fill the dead tissue wound space requires a 24 times oxidative burst. This intense competition for oxygen can cause inflammation and swelling. HBOT provides this additional oxygen.

* HBOT sessions prior to surgery will eliminate the reperfusion phenomenon, reducing the chance of scar tissue forming. It also reduces inflamation and swelling.

* Hypoxia in normally perfused tissue can unexpectedly occur post-surgically due to an inflammatory response and edema. HBOT corrects hypoxia and dramatically reduces inflammation and swelling.

* HBOT increases fibroblast replication and collagen production. It also raises the RNA/DNA ratio in the tissues, indicating increased formation of rough endoplasmic reticulum of cells of the wounded area.

* From Beverly Hills to New York City, more and more cosmetic surgeons are adding Hyperbaric Oxygen Therapy to their protocol, routinely treating their Patients before and after surgery, sometimes offering hyperbaric facilities in the offices of their rights

O3 Ozone in Dermatology: Myth or Reality

Posted by: Lubov Pavlova in Articles
S.L.Krivatkin, E.V.Krivatkina
Nizhny Novgorod, Russia

The use of medical ozone in the treatment of human diseases has more than 80 year history.

Ozonetherapy is actively used in Europe (Austria, Germany, Italy, Russia, France, Switzerland etc) as well as in Latin America (on Cuba and in Brazil), great interest to this method is shown in USA.

The spectrum of ozone applications in the medicine is so wide, that with some skepticism it is considered a panacea for all diseases.

The universality of ozone therapy can be explained by scientifically verified breadth of biological action of ozone related to its physical-chemical properties which are responsible for its bactericidal, virucidal, fungicidal, anti-inflammatory, microcirculation stimulating, immunomodulating and other therapeutical effects. The use of ozone in dermatology, in the field of medicine including more than 2000 nosological units has its own features.

Our 8 year experience of ozonetherapy in dermatosis confirms the existence of high-effective alternative therapeutic method in dermatological practice.

We observed a group of ambulant patients (n = 495) with herpes (99), acne (75), eczema (64), pyoderma (61), alopecia (42) as well as shank venous ulcer, lichen planus, neurodermitis, localized scleroderma, psoriasis and psoriatic arthritis and other, less frequent dermatosis.

The following traditional methods of ozonetherapy were used: minor ozonated autohemotherapy, rectal ozone insufflations, intramuscular ozone injections, external applications of ozonized olive oil and ozone-oxygen mixture by means of plastic bags.

The most used method is minor autohemotherapy owing to the beneficial relationship between the efficiency and simplicity/convenience of application. The treatment should be peformed daily or 2-3 times a week in a cycle of 3-4 to 15-20 procedures. Ozonetherapy is usually used as a monotherapy, rarely in combination with traditionally used external medicines.

The therapy used showed the following results: disappearance of clinical picture of dermatosis or significant improvement was observed in all the patients with herpes, 95% - with pyoderma, 75% - with eczema, 2/3 ?? � with alopecia, lichen planus and neurodermitis, 3/5 ??� with psoriasis, 2/5 ??� with acne, localized scleroderma and shank venous ulcer.

The best therapeutical results were received when ozonetherapy was started at the beginning of disease or in acute phase. So, ozonetherapy proved to be particularly effective in the patients with localized scleroderma at first appearance of disease or its aggravation (clinical picture of purple ring and erythema in focuses) and with disease duration less than 3 years.

The same is about the treatment of lichen planus (the absolutely best results in acute forms). The appearance of clear inflammatory symptoms in the focuses before the treatment means, as a rule, the efficiency of ozonetherapy. For example, the acute inflammatory forms of acne appear to be the most favourable indication for ozonetherapy.

However, ozonetherapy was not enough effective in lightly inflammatory forms of acne, severe forms of neurodermitis, verrucous forms of lichen planus.

In some dermatosis a repeated course of ozonetherapy was required (alopecia, neurodermitis, localized scleroderma etc).

In cases when ozonetherapy failed the patients had severe associated pathologies. So, ozonetherapy did not show positive results by treating the woman, 65 years old, suffering from bullous forms of localized scleroderma at presence of associated urolithiasis, ischemic heart disease and gastroduodenitis.

Ozonetherapy failed in the patients with alopecia areata after or at presence of severe associated diseases (two patients with craniocerebral injury trauma, other two patients with pneumonia and Besedow?? �s disease).

Ozonetherapy provided enough stable remote results: in absolute majority the patients showed longer lasting remission time after ozonetherapy than after traditionally used methods of treatment.

Ozonetherapy is good tolerated by patients: the frequency of side effects is less than 1% (allergic dermatitis). After all, ozonetherapy is a kind of soft therapy with its advantages and disadvantages: it is not so effective as cytostatics or corticosteroids, but does not cause possible fatal side-effects of the last medicines.

So, having taken into account sufficient efficiency of ozonetherapy in some dermatosis, simple performance in ambulant practice and safety, cheapness of raw materials (oxygen), we are sure that in the near future ozonetherapy will be a very popular alternative method of treatment of skin diseases.

Owing to the known mechanisms of action of ozone, ozonetherapy will prove to be effective in other dermatosis caused by virusal, bacterial or fungal agents as well as at predominance of inflammatory elements, microcirculatory disturbances and immunity disorders in the pathogenesis of disease.

The ozonized oils of vegetal source constitute an appropriate method for the local therapy of several diseases. The interaction of ozone with these oils produce a mixture of chemical compounds (ozonides and peroxides) with a great germicide power.

The results of topical applications of ozonized oil (OLEOZON®) in dermatological diseases of viral, fungal and bacterial origin, during 9 years of study, were presented. Among the diseases of bacterial origin (for example, pyoderma), 600 patients were treated, obtaining a healing criterion in 87 %.

According to mycotic diseases (for example, epidermophytosis, onychomycosis, etc), 1000 patients received the treatment, with 91 % of healing. In viral diseases (for example, herpes simplex), 300 patients were treated and in 72 % the relapses disappeared. Any side effect was observed.

A preliminary study was carried out with the application of ozonized cream to fifty patients carriers of vulgar acne in different stages of evolution.

Very good results were obtained with regression of the eriteaatopustule scabby lesions. This allowed these patients to have a satisfactory result in relation to other therapies applied in this dermatosis. We consider this treatment is one more choice in the therapy for acne.

Simplex herpes is a skin disease of viral etiology of difficult treatment because its recurrence, and its repeated out breaks, with lesion of erythema and vesicles, aggregated in clusters; and symptoms like burn and prurito.

After the three first days, the virus penetrates into the groups of ganglious near the lesion. It makes very difficult the recovery with the traditional treatment, because of the frequent recurrence. The treatment with subcutaneous ozone has avoided the relapses with positive results.

The research was made in a group of 84 patients with simplex herpes localized in the genitals, lips and gluteals. It has not been reported the antiviral effect of ozone in this pathology by a subcutaneous way. This make the treatment very new and safe.

S.L. Krivatkin, E.V. Krivatkina.
Sormovo Interdistric Dermatovenereological Dispensary, Nizhny Novgorod, Russia.

Ozone is one of a new therapeutic agents being used in dermatology. In a recent test, 64 patients (41 men and 23 women aged 15-64) suffering from different forms of pyoderma (ecthyma-6, erysipelas-1, furunculosis-18, hidradenitis-2, impetigo-29 and sycosis barbae-8) received ozone therapy (OT).

Two of its standard forms were applied: autohemotherapy minor (AHT min, intramuscular injections of 10 mL 02/03 mixture in 5 mL of autoblood) and techniques with the use of a plastic bag. As a source of 02/03 mixture the ozonizer was used. The concentration of ozone was 7-20 µg/mL. Along with OT most of the patients used traditional ointments.

Clinical recovery was achieved in 51 patients, considerable improvement in 10, improvement in 1 and no positive results in 2 patients. These data compared with the results of the previous traditional treatment (ex. systemic antibiotics) in the same 20 patients showed a better and quicker improvement in most of them.

Ozone therapy was well tolerated. It is safe to say that among the advantages of OT there are good tolerance, as well as the possibility of use in patients with candidiasis and intolerance to antibiotics, absence of pathogen resistance and relative inexpensiveness.

Howerver at this time, OT can not ensure quick results in severe pyoderma (ecthyma, etc.) when systemic antibiotics can.

Thus ozone therapy is an effective, safe and inexpensive approach to the treatment of pyoderma, it is worthy to be used.

EXPERIENCES WITH O3 OZONE THERAPY IN THE SUTTON DISEASE (PERIADENITIS MUCOUS NECROTICA RECURRENS). A CASE REPORT.

Sutton disease (Periadenitis Mucous Necrotic Recurrent) is a rather rare pathological condition characterized by several ulceration of the mouth, very painful, to a tendency to relapse and with a retracted healing. Many factors are involucrated in the pathogenesis of this disease, but the immgical response is one of the main aspects considered in the evolution of the pathological process.

It is presented a case of a 12-year-old age patient with diagnosis of Sutton disease with 5 years of evolution, without satisfactory results, after testing multiple therapeutic methods. The patient has been treated with ozone therapy by gas rectal insufflation (20 sessions) and local applications of ozonized sunflower oil (OLEOZON) during 2 years.

Relapses decrease during the treatment and the healing of the ulcerous injuries was more rapid and scar retractions was not observed. It is made a wide review of the topic in order to justify the application of this therapeutic method. This is the first report using ozone therapy treatment in this disease.

OZ-P-112: THE O3 OZONE IN SOME SKIN VIROSES.

Medical Surgical Research Center

The skin conditions are caused by viral infection in a large number of patients in the general population and in our environment is observed frequently. Different authors agree that in many of these patients there is a depression in the immune system. Knowing that ozone addition to the immunomodulatory effects virucidal action was carried out this study, 84 patients who were treated with ozone in different ways.

All cases diagnosed as herpes simplex were classified according to the location of the lesion, genital sores, and buttocks, using the subcutaneous route simultaneously with topical application of ozonated oil, and herpes zoster (20 patients) was used only intramuscularly.

We propose that ozone is effective in treating these diseases with satisfactory results especially in recurrent herpes simplex that administering subcutaneous ozone is able to control the disease.

OZ-P-132: HEALTH RECORD ON APPLICATION OF ozonized sunflower oil O3 "OLEOZON ® "IN THE epidermophytosis.

The vegetable oils have become means for the treatment with ozone. To ozonate these oils results in a series of chemical compounds which possess a germicidal character. The aim of our study was to obtain the sanitary registration of ozonized sunflower oil OLEOZON ® for the treatment of epidermophytosis.

The culmination of veterinary led several steps: 1-Chemical-Pharmaceutical Studies (assembly and development of different techniques, study of stability, etc..). 2-Toxicological (irritability dermal phototoxicity, sensitization test, acute toxicity and subchronic dermal, genotoxic evaluation). 3-Pharmacological studies (antifungal and antibacterial activity, effect on dermatomycosis of the rabbit, healing action).

They conducted a randomized controlled phase III, randomized, open, which compared the OLEOZON ® with nizoral, in patients with epidermophytosis. Group A (100 patients) received topical application of OLEOZON ® , 2 times a day for six weeks. Group B received treatment with Nizoral, the same number of times and for a like period of time that the above product. Efficacy was evaluated clinically (disappearance of all lesions, with or without mycological examination negativity) and microbiologically (microbiolólgico test negativity).

The overall assessment of the study, after 6 weeks of treatment, showed 75 and 81% efficiency for OLEOZON ® and Nizoral, respectively, without significant differences between groups. There were no cases of infection on added or adverse reactions.

One may note that both drugs have equal efficacy in the treatment of epidermophytosis. Registration was obtained OLEOZON ® for the treatment of epidermophytosis the May 12, 1999 with registration number 1498.

OZ-P-136: A SOLUTION TO THE FOOT epidermophytosis.

Teaching HospitaL,
Ozone Research Center.

The dermatoficias fungal infections are sometimes resistant to treatment and enrolled in many cases with relapses, causing limitations and disability. Imidazole derivatives, broad-spectrum antifungal, expensive, therefore the search for new drugs for this disease still remains.

Thanks to the fungicidal effect of ozonized sunflower oil (OLEOZON ® ) presents its application in the treatment of epidermophytosis feet, whose goal was to generalize its use in patients with this condition treated at the Hospital "Carlos J. Finlay "in 1998-1999, from outpatient medical examinations for health screening, medical fees and entered.

504 patients were treated, curing 437 patients (86.7%). The most effective response, according to the clinical form was the scaly and macerated. There were no adverse effects.

OZ-P-137: TREATMENT OF OIL O3 ozonized pyoderma.

Teaching Hospital,
Ozone Research Center.

The pyoderma are skin conditions caused by pyogenic bacteria corresponding to 90% to Staphylococcus aureus coagulase positive and Streptococcus beta haemolyticus . Based on the best results obtained in preliminary studies with ozonized oil compared with neomycin ointment in primary and secondary pyoderma, a decision is widely applied.

1098 patients were treated for pyoderma (1995 to 1999) with ozonized oil twice a day for 10 days. Healing was obtained in 997 patients (90.8%) without adverse effects.

OZ-P-138: IMPLEMENTATION OF O3 OZONE IN HIGH pyoderma.

Clinic Natural and Traditional Medicine.

The ozone-oxygen mixture has demonstrated bactericidal, fungicidal and virucidal, being useful for treating diseases in which bacteria are involved. For this reason, we conducted a prospective open clinical trial in 190 patients with secondary piodermites diagnosed clinically and confirmed by the isolation of morphogenera and species of the causative agents by bacteriological examinations. These patients were selected outpatients between 1996 and 1997, randomly assigning them simple, without replacement, two treatment groups.

Group 1 was applied fomentations of potassium permanganate (to 1 x 2 000) for 15 min three times a day and alibour cream.

Group 2 was applied building ozonated water for 15 min three times a day. In both groups, treatment lasted 3 weeks, with weekly assessments for 21 d.

The results showed a better response in the group treated with ozonated water, yielding a 92.9% efficiency and a higher cure rate in the first week. There were no adverse reactions to ozonated water.

A.I. Kuzmichev, Ja.F. Kutasevitch, E.A. Petrusenko, V.D. Tsydeiko
Ukrainian SRI of Dermatology and Venereology, Kharkov, Ukraine Kiev Polytechnical
Institute, Kiev, Ukraine

The efficiency of low ozone concentrations using on patients (53 men) with different dermatitis was studied. The ozone/oxygen (or ozone/air) mixtures were used for treatment of sores in the sealed polymer chamber.

The ozone concentration was 10-50 mg/m 3; the procedure duration was 10 min for the ozone/oxygen mixture and 30 min for the ozone/air mixture. It was enough to make 3-8 procedures for the visible effect of ozone. The obtained results allow considering ozone therapy as the perspective method for dermatology

O3 Ozone Therapy in Cosmetology

HBOT / O2 Oxygen Provides Dramatic Improvements for Cosmetic Surgeons

O3 Ozone in Dermatology: Myth or Reality